小鼠胰岛素样生长因子1(IGF-1)ELISA试剂盒-武汉恩玑生命科技有限公司

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小鼠胰岛素样生长因子1(IGF-1)ELISA试剂盒

品牌：EnkiLife

产地：中国

型号：96T

货号：EM10630

价格： ￥2800/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

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产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EM10630
用途
检测方法	夹心法
保质期
适应物种	Mouse
检测限	9.37ng/mL
数量
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Mouse IGF-1抗体包被于酶标板上,实验时样品(或标准品)中的Mouse IGF-1会与包被抗体结合。后依次加入生物素化的抗Mouse IGF-1抗体和HRP酶标记的亲和素,抗Mouse IGF-1抗体与结合在包被抗体上的Mouse IGF-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Mouse IGF-1浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Mouse IGF-1的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Insulin-like Growth Factor 1
Synonym	IGF1;IGFI;IGF-I;IGF1A;IGF-IA;IGF-IB;MGF;Somatomedin C;
Assay Type	Sandwich
Reactivity	Mouse
Range	15.62-1000ng/mL
Sensitivity	9.37ng/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	P05017
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Mouse IGF-1 were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Mouse IGF-1 were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	53.38	126.54	361.39	51.8	128.52	350.27
Standard deviation	3.67	7.19	13.12	3.04	5.62	16.39
CV (%)	6.88	5.68	3.63	5.86	4.37	4.68

◤回收率(Recovery):

Three different levels of Mouse IGF-1 spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-101	95
EDTA plasma (n=8)	86-100	93
Cell culture media (n=8)	89-102	94

◤组份清单(Component List):

Component	Specifications
Mouse IGF-1 Microplate	96T: 8 wells ×12 strips
Mouse IGF-1 Microplate	48T: 8 wells ×6 strips
Mouse IGF-1 Standard	96T: 2 vial
Mouse IGF-1 Standard