小鼠血管内皮细胞生长因子A(VEGF-A)ELISA试剂盒-武汉恩玑生命科技有限公司

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小鼠血管内皮细胞生长因子A(VEGF-A)ELISA试剂盒

品牌：EnkiLife

产地：中国

型号：96T

货号：EM10657

价格： ￥2800/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

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产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EM10657
用途
检测方法	夹心法
保质期
适应物种	Mouse
检测限	18.75pg/mL
数量
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Mouse VEGF-A抗体包被于酶标板上,实验时样品(或标准品)中的Mouse VEGF-A会与包被抗体结合。后依次加入生物素化的抗Mouse VEGF-A抗体和HRP酶标记的亲和素,抗Mouse VEGF-A抗体与结合在包被抗体上的Mouse VEGF-A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Mouse VEGF-A浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Mouse VEGF-A的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Vascular Endothelial Cell Growth Factor A
Synonym	VEGF-A;VEGFA;MVCD1;VPF;
Assay Type	Sandwich
Reactivity	Mouse
Range	31.25-2000pg/mL
Sensitivity	18.75pg/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	Q00731
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Mouse VEGF-A were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Mouse VEGF-A were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	95.56	313	772.55	90.88	305.9	826.11
Standard deviation	5.92	13.15	35.77	5.18	16.7	39.9
CV (%)	6.2	4.2	4.63	5.7	5.46	4.83

◤回收率(Recovery):

Three different levels of Mouse VEGF-A spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-98	91
EDTA plasma (n=8)	85-97	92
Cell culture media (n=8)	92-106	99

◤组份清单(Component List):

Component	Specifications
Mouse VEGF-A Microplate	96T: 8 wells ×12 strips
Mouse VEGF-A Microplate	48T: 8 wells ×6 strips
Mouse VEGF-A Standard	96T: 2 vial
Mouse VEGF-A Standard