小鼠干扰素-γ受体1(IFN-γ R1)ELISA试剂盒-武汉恩玑生命科技有限公司

产品展厅

小鼠干扰素-γ受体1(IFN-γ R1)ELISA试剂盒

品牌：EnkiLife

产地：中国

型号：96T

货号：EM10663

价格： ￥2800/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

发送咨询信息

产品详请

产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EM10663
用途
检测方法	夹心法
保质期
适应物种	Mouse
检测限	46.87pg/mL
数量
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Mouse IFN-γ R1抗体包被于酶标板上,实验时样品(或标准品)中的Mouse IFN-γ R1会与包被抗体结合。后依次加入生物素化的抗Mouse IFN-γ R1抗体和HRP酶标记的亲和素,抗Mouse IFN-γ R1抗体与结合在包被抗体上的Mouse IFN-γ R1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Mouse IFN-γ R1浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Mouse IFN-γ R1的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Interferon Gamma Receptor 1
Synonym	Interferon gamma receptor 1;IFNGR1;INGR1;IFN-gamma receptor 1;IFN-gamma-R1;CDw119;Interferon gamma receptor alpha-chain;IFN-gamma-R-alpha;CD119;
Assay Type	Sandwich
Reactivity	Mouse
Range	78.12-5000pg/mL
Sensitivity	46.87pg/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	P15261
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Mouse IFN-γ R1 were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Mouse IFN-γ R1 were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	255.97	460.25	2493.4	262.81	421.36	2358.74
Standard deviation	14.85	27.38	89.51	17.87	17.91	88.69
CV (%)	5.8	5.95	3.59	6.8	4.25	3.76

◤回收率(Recovery):

Three different levels of Mouse IFN-γ R1 spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	84-99	91
EDTA plasma (n=8)	95-107	101
Cell culture media (n=8)	91-104	96

◤组份清单(Component List):

Component	Specifications
Mouse IFN-γ R1 Microplate	96T: 8 wells ×12 strips
Mouse IFN-γ R1 Microplate	48T: 8 wells ×6 strips
Mouse IFN-γ R1 Standard