人肿瘤坏死因子α(TNF-α)ELISA试剂盒-武汉恩玑生命科技有限公司

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人肿瘤坏死因子α(TNF-α)ELISA试剂盒

英文名称：TNF-α (Human) ELISA Kit

品牌：EnkiLife

产地：中国

型号：96T

货号：EH10021

价格： ￥2200/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

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产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EH10021
用途
检测方法	夹心法
保质期
适应物种	Human
检测限	4.68pg/mL
数量
英文名称	TNF-α (Human) ELISA Kit
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Human TNF-α抗体包被于酶标板上,实验时样品(或标准品)中的Human TNF-α会与包被抗体结合。后依次加入生物素化的抗Human TNF-α抗体和HRP酶标记的亲和素,抗Human TNF-α抗体与结合在包被抗体上的Human TNF-α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Human TNF-α浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Human TNF-α的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Tumor Necrosis Factor Alpha
Synonym	DIF;TNF-alpha;TNFA;TNFSF2;
Assay Type	Sandwich
Reactivity	Human
Range	7.81-500pg/mL
Sensitivity	4.68pg/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	P01375
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Human TNF-α were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Human TNF-α were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	22.81	63.89	177.83	20.97	62.3	171.09
Standard deviation	1.33	3.23	5.42	1.07	2.64	8.93
CV (%)	5.84	5.05	3.05	5.09	4.24	5.22

◤回收率(Recovery):

Three different levels of Human TNF-α spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	93
EDTA plasma (n=8)	93-107	99
Cell culture media (n=8)	90-101	96

◤组份清单(Component List):

Component	Specifications
Human TNF-α Microplate	96T: 8 wells ×12 strips
Human TNF-α Microplate	48T: 8 wells ×6 strips
Human TNF-α Standard	96T: 2 vial
Human TNF-α Standard