人基质金属蛋白酶9(MMP-9)ELISA试剂盒-武汉恩玑生命科技有限公司

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人基质金属蛋白酶9(MMP-9)ELISA试剂盒

英文名称：MMP-9 (Human) ELISA Kit

品牌：EnkiLife

产地：中国

型号：96T

货号：EH10079

价格： ￥2200/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

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产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EH10079
用途
检测方法	夹心法
保质期
适应物种	Human
检测限	0.09ng/mL
数量
英文名称	MMP-9 (Human) ELISA Kit
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Human MMP-9抗体包被于酶标板上,实验时样品(或标准品)中的Human MMP-9会与包被抗体结合。后依次加入生物素化的抗Human MMP-9抗体和HRP酶标记的亲和素,抗Human MMP-9抗体与结合在包被抗体上的Human MMP-9结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Human MMP-9浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Human MMP-9的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Matrix Metalloproteinase 9
Synonym	MMP9;CLG4B;Gelatinase B;GELB;MANDP2;92kDa Type IV Collagenase;92 KDa Gelatinase;
Assay Type	Sandwich
Reactivity	Human
Range	0.15-10ng/mL
Sensitivity	0.09ng/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	P14780
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Human MMP-9 were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Human MMP-9 were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.54	1.46	3.46	0.52	1.58	3.49
Standard deviation	0.03	0.07	0.13	0.03	0.07	0.19
CV (%)	6.24	4.59	3.71	6.13	4.57	5.49

◤回收率(Recovery):

Three different levels of Human MMP-9 spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-104	97
EDTA plasma (n=8)	85-100	91
Cell culture media (n=8)	89-100	94

◤组份清单(Component List):

Component	Specifications
Human MMP-9 Microplate	96T: 8 wells ×12 strips
Human MMP-9 Microplate	48T: 8 wells ×6 strips
Human MMP-9 Standard	96T: 2 vial
Human MMP-9 Standard