人胱抑素C(Cys-C)ELISA试剂盒-武汉恩玑生命科技有限公司

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人胱抑素C(Cys-C)ELISA试剂盒

英文名称：Cys-C (Human) ELISA Kit

品牌：EnkiLife

产地：中国

型号：96T

货号：EH10143

价格： ￥2200/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

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产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EH10143
用途
检测方法	夹心法
保质期
适应物种	Human
检测限	0.18ng/mL
数量
英文名称	Cys-C (Human) ELISA Kit
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Human Cys-C抗体包被于酶标板上,实验时样品(或标准品)中的Human Cys-C会与包被抗体结合。后依次加入生物素化的抗Human Cys-C抗体和HRP酶标记的亲和素,抗Human Cys-C抗体与结合在包被抗体上的Human Cys-C结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Human Cys-C浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Human Cys-C的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Cystatin C
Synonym	CST3;Cystatin 3;ARMD11;Post-Gamma-Globulin;
Assay Type	Sandwich
Reactivity	Human
Range	0.31-20ng/mL
Sensitivity	0.18ng/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	P01034
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Human Cys-C were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Human Cys-C were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.93	2.02	8.39	0.88	1.89	8.13
Standard deviation	0.05	0.1	0.36	0.05	0.1	0.29
CV (%)	5.53	5.16	4.26	5.8	5.16	3.58

◤回收率(Recovery):

Three different levels of Human Cys-C spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-103	96
EDTA plasma (n=8)	86-98	91
Cell culture media (n=8)	85-96	90

◤组份清单(Component List):

Component	Specifications
Human Cys-C Microplate	96T: 8 wells ×12 strips
Human Cys-C Microplate	48T: 8 wells ×6 strips
Human Cys-C Standard	96T: 2 vial
Human Cys-C Standard