人血管内皮生长因子165(VEGF165)ELISA试剂盒-武汉恩玑生命科技有限公司

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人血管内皮生长因子165(VEGF165)ELISA试剂盒

英文名称：VEGF165 (Human) ELISA Kit

品牌：EnkiLife

产地：中国

型号：96T

货号：EH10297

价格： ￥2200/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

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产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EH10297
用途
检测方法	夹心法
保质期
适应物种	Human
检测限	18.75pg/mL
数量
英文名称	VEGF165 (Human) ELISA Kit
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Human VEGF165抗体包被于酶标板上,实验时样品(或标准品)中的Human VEGF165会与包被抗体结合。后依次加入生物素化的抗Human VEGF165抗体和HRP酶标记的亲和素,抗Human VEGF165抗体与结合在包被抗体上的Human VEGF165结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Human VEGF165浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Human VEGF165的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Vascular Endothelial Growth Factor 165
Synonym
Assay Type	Sandwich
Reactivity	Human
Range	31.25-2000pg/mL
Sensitivity	18.75pg/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	P15692
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Human VEGF165 were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Human VEGF165 were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	91	228.31	760.92	95.04	214.74	764.27
Standard deviation	4.91	11.76	39.04	6.19	11.27	23.54
CV (%)	5.4	5.15	5.13	6.51	5.25	3.08

◤回收率(Recovery):

Three different levels of Human VEGF165 spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-108	99
EDTA plasma (n=8)	87-98	92
Cell culture media (n=8)	93-107	100

◤组份清单(Component List):

Component	Specifications
Human VEGF165 Microplate	96T: 8 wells ×12 strips
Human VEGF165 Microplate	48T: 8 wells ×6 strips
Human VEGF165 Standard	96T: 2 vial
Human VEGF165 Standard