人甲胎蛋白(αFP)ELISA试剂盒-武汉恩玑生命科技有限公司

产品展厅

人甲胎蛋白(αFP)ELISA试剂盒

英文名称：αFP (Human) ELISA Kit

品牌：EnkiLife

产地：中国

型号：96T

货号：EH10304

价格： ￥2200/盒
发布日期： 2024-12-03
更新日期： 2025-05-22

发送咨询信息

产品详请

产地	中国
保存条件	按照说明书存放
品牌	EnkiLife
货号	EH10304
用途
检测方法	夹心法
保质期
适应物种	Human
检测限	0.93ng/mL
数量
英文名称	αFP (Human) ELISA Kit
包装规格	96T
标记物
样本
应用
是否进口	否

◤反应原理(Test Principle):

本试产品采用双抗体夹心ELISA法。用抗Human αFP抗体包被于酶标板上,实验时样品(或标准品)中的Human αFP会与包被抗体结合。后依次加入生物素化的抗Human αFP抗体和HRP酶标记的亲和素,抗Human αFP抗体与结合在包被抗体上的Human αFP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Human αFP浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Human αFP的浓度。

◤存储(Storage):

Store the product at 2-8°C before unopened.

Upon receipt, unpack promptly and store as recommended in the instructions.

◤关键信息(Key Features):

Full Name	Alpha-Fetoprotein
Synonym	AFP;FETA;HPAFP;Alpha-fetoglobulin;oralpha fetal protein;
Assay Type	Sandwich
Reactivity	Human
Range	1.56-100ng/mL
Sensitivity	0.93ng/mL
Sample Type	Serum,plasma and other biological fluids
Sample volume	100μL
UNIProt ID	P02771
Microplate	96-wells plate breakable into 12 x 8 wells strip

◤实验过程(Assay Procedures):

◤精密度(Precision):

Intra-assay precision (intra-assay precision): Low, medium, and high levels of Human αFP were measured on a single plate 20 times at each level.

Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Human αFP were assayed 20 times per plate on 3 different plates.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.63	15.87	49.53	4.55	14.44	44.82
Standard deviation	0.3	0.81	1.5	0.3	0.72	1.71
CV (%)	6.48	5.09	3.03	6.6	5.02	3.81

◤回收率(Recovery):

Three different levels of Human αFP spiked in samples were evaluated for recovery in different matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	93
EDTA plasma (n=8)	84-99	91
Cell culture media (n=8)	86-98	93

◤组份清单(Component List):

Component	Specifications
Human αFP Microplate	96T: 8 wells ×12 strips
Human αFP Microplate	48T: 8 wells ×6 strips
Human αFP Standard	96T: 2 vial
Human αFP Standard